Proximate composition of small indigenous fish species of Budhi Rapti river Chitwan Nepal

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Department of Zoology
Abstract
Small indigenous fish are delicious than other fish used for food. An attempt has been undertaken in the current study to determine the proximate composition in small indigenous fish species of Budhi Rapti River Chitwan Nepal. The goal of the current study is to determine the proximate composition of the five local small indigenous fish species (Puntius ticto, Puntius conchonius, Mystus tengara, Barilius bendelisis, Acanthocobitis botia) found in Budhi Rapti River Chitwan Nepal. Samples were collected between January to March 2022. The samples were cleaned, gutted, dried, and grinded to obtain a homogenized powder that was then maintained in plastic containers for chemical analysis. Proximate analysis was performed in the laboratory of National Animal Nutrition Research Centre (NARC). For proximate analysis Association of Official Analytical Chemists (AOAC) method was used. Major proximate compositions such as, moisture, protein, lipid, ash, and fiber were estimated. Proximate compositions were found varied among the species. Protein was estimated in Puntius ticto (54.77 %), Puntius conchonius (63.95 %), Mystus tengara (70.68%), Barilius bendelisis (69.82), Acanthocobitis botia (67.98%) respectively. The results show the highest moisture content in Mystus tengara (99%) and lowest in Puntius ticto (98.17%). The Lipid content in the present study varied between 9.05 - 15.65% with the highest in Acanthocobitis. botia (15.65%) and lowest in Puntius. chonchonius (9.05%). The crude fiber content varied between (1.17 -7.42) with the highest in Barilius bendelisis (7.42%) and lowest in Puntius ticto. The ash content ranged between 16.54 -24.02% with the highest in Puntius conchonius (24.02%) and lowest in Barilius bendelisis (16.54%). Proximate analysis was performed in National Animal Nutrition Research Centre, Khumaltar. Analysis was carried out in triplicate. These findings demonstrate that all these fishes have a significant amount of nutrient. At the end of the study, no significant difference was observed in moisture, but protein, lipid, Fibre, and ash content shows significant differences. It is clear from the current study that these SIS are a reliable supply of macronutrients, protecting both dietary and financial security of Chitwan, Nepal.
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