ANTIBIOTIC SUSCEPTIBILITY TESTING OF ISOLATED Staphylococcus aureus FROM PANEER SOLD IN KATHMANDU
Date
2023-08-17
Authors
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Publisher
Amrit Campus
Abstract
Paneer is a fresh acid-set, non-aged, non-melting soft indigenous food made from
curdling milk. Safety of dairy products is an issue of public health in Nepal.
Staphylococcus aureus is an food borne pathogen of importance in dairy products. The
main aim of this study is to detect microbial quality of paneer marketed in different
localities of Kathmandu district. In present study, 30 paneer samples were randomly
collected from dairy shops from 5 different locations in Kathmandu (Bafal, Kalanki,
Naikap, Sitapaila and Thamel) during April to May 2022 and processed at laboratory of
of Microbiology, Amrit Campus. Microbial analysis of paneer was done by Total
bacteria count and total Staphylococcal count S. aureus were identified by biochemical
tests and their antibiotic susceptibilty test was done by Kirby-Bauer disc diffusion
method. Results obtained from this investigation revealed that out of 30 samples, 39.9%
in Total Bacterial Count (TBC) was above specification. Among 30 samples, 29 samples
showed Staphylococcus spp. growth and of those only 12 confirmed as Staphylococcus
aureus . Among them 6 (20 %) exceeded specification of 100 cfu/gm. Antimicrobial
susceptibility pattern of S. aureus showed 100% sensitivity towards Cotrimoxazole and
Gentamycin and Levofloxacin, whereas resistance towards Chloramphenicol (8.3%),
Cefoxitin (8.3%), Tetracycline(8.3%), Penicillin G (41.6%), Ampicillin (16.6%),
Nalidixic acid (41.6%) and Azithromycin (16.6%). MRSA was detected in 1(3.34%).
This study concluded that maximum number of sample exceeding microbial specification
and antibiotic resistant isolates were reported from Naikap. Irresponsible and over use of
antibiotics has lead to an increased the presence of multidrug Regular microbial quality
monitoring of marketed paneer should be carried out by authorized Institution of
Government of Nepal. Therefore, it is necessary to monitor and control the quality
marketed in Kathmandu.
Description
Keywords
Ampicillin, Total bacterial count, Staphylococcus aureus, Paneer, Cefoxitin, Antimicrobial susceptibility test