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Browsing Syllabus by Author "Banjade, Purnima"

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    ANTIOXIDANT AND BIOLOGICAL EVALUATION OF METHANOLIC, ETHYL ACETATE AND DICHLOROMETHANE EXTRACT OF Zingiber officinale COLLECTED FROM TWO DIFFERENT DISTRICTS OF NEPAL
    (Department of Chemistry, Birendra Multiple Campus, 2025-02-06) Banjade, Purnima; Ganga Raj Pokhrel, Ph.D
    Antioxidant and biological evaluation of methanolic, ethyl acetate, and dichloromethane extract of Zingiber officinale collected from two different districts of Nepal is not clearly understood yet. This study investigates these properties in ginger extracts from Arghakhanchi and Dang districts. The ginger samples were gathered, dried, and extracted using the cold percolation method with three solvents: methanol (MeOH), dichloromethane (DCM), and ethyl acetate (EtOAc). Total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity were measured via DPPH assay, antimicrobial activity, and toxicity using a brine shrimp bioassay. The methanolic extract from Dang district (S2(MeOH)) showed the highest TPC (123.02 ± 2.84 mg GAE/g) and TFC (25.42 ± 1.69 mg QE/g), with strong antioxidant activity (IC50 = 162.73 ± 3.22 μg/mL). The dichloromethane extract from Arghakhanchi (S1(DCM)) had the lowest TPC (36.23 ± 3.45 mg GAE/g) and TFC (14.32 ± 1.74 mg QE/g), with weaker antioxidant activity (IC50 = 279.63 ± 14.67 μg/mL). The ethyl acetate extract from Dang (S2(EtOAc)) showed the best antimicrobial activity, especially against S. aureus and P. aeruginosa. The methanolic extract demonstrated strong antifungal properties. Brine shrimp bioassay results indicated higher toxicity in the methanolic extract (LC50 = 3.0 × 10³ μg/mL). Ginger from Dang district showed the most promising antioxidant and antimicrobial properties. Further research on active compounds is recommended for potential medicinal use. Keywords: Antimicrobial, Antioxidant, Ginger (Zingiber officinale), Total Flavonoid Content, Total Phenolic Content.