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Item NUTRIENT ELEMENTS AND HR-LCMS ANALYSIS OF SECONDARY METABOLITES PRESENT IN METHANOLIC EXTRACTS OF Colocasia esculenta (L.) SCHOTT (cocoyam)(Department of Chemistry, Birendra Multiple Campus, 2025-02-06) MAHATO, AJAYA; Dr. Ganga Raj PokhrelNutrient Elements and HR-LCMS Analysis of Secondary Metabolites Present in Methanolic Extracts of (Colocasia esculenta (L.) Schott (Cocoyam) is still not understood clearly. Methanolic extracts of three different parts (tuber, petiole and leaf) of plants from different sampling spots (Palung, Upperdangadhi and Padampur) were utilized to assay antioxidants activities through 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and phosphomolybdate assay (TAA), total phenolic (TPC), total flavonoid (TFC) and total carbohydrate content (TCC) using spectrophotometer (T80+, PG Instruments, UK). The antimicrobial susceptibility test of methanolic extracts of plant samples was conducted on seven microorganisms i.e. E. coli, K. pneumonia, P. aeruginosa, B. subtilis, S. aureus, A. baumannii and S. typhi, Pseudomonas and Salmonella species. Nutrient elements and toxic metals quantification and HR-LCMS analysis of plant tuber, petiole and leaf from Upperdangadhi were carried out at Centre for Research in Nanotechnology and Science (CRNTS), Sophisticated Analytical Instrument Facility (SAIF), Indian Institute of Technology, Bombay, 400076, India, using ICP-AES and HR-LCMS spectroscope model G6550A respectively.IC50 value of plant extracts was found in between 240.45-1018.44 µg/mL. TAA by phosphomolybdate assay was found 1.25±0.01 to 28.29±0.01 mg AAE/g DS. TPC was found in between 6.38±0.004 and 19.96±0.01 mg GAE/g DS. TFC was found in the range of 10.46±0.0007 and 89.1±0.006 mg QE/g DS. TCC was found in between 1.78±0.0 and 112±0.0 mg GE/g DS. Concentrations of elements were found in the order of tuber, petiole and leaf as: Ca (8910.0, 303.0, 355.0 mg/Kg), Cr (0.476, 0.252, 0.427 mg/Kg), Cu (1.226, 0.670, 0.727 mg/Kg), Fe (15.6, 1.241, 2.41 mg/Kg), Mg (96.8, 43.6, 104.0 mg/Kg), Zn (128.0, 0.685, 1.942 mg/Kg) and As (15.7, 9.8, 65.6 mg/Kg). Concentration of Pb was below the detection limit(1ppb).HR-LCMS analysis of tuber, petiole and leaf revealed that the extract contained 200, 140 unidentified, 60 identified- 11 alkaloids, 4 phenolic, 5 flavonoids, 3 glycosides, 5 steroids, 32 others, 200, 85 unidentified, 115 identified- 12 alkaloids, 15 phenolic, 26 flavonoids, 9 glycosides, 53 others, 197, 106 unidentified, 91 identified- 13 alkaloids, 17 flavonoids, 3 terpenoids, 1 steroid, 12 pharmaceutical drugs, 45 others respectively. Neuraminic acid, Amprenavir, Butin, catechin, cyclopamine,disopyramide, Funtumine, gentamycin, Jervin, Kaemferol, ketamine, retapamulin, swertiamarin, syringic acid, usambarensine etc. are potentially bioactive. Key words: Cocoyam, Phytochemical screening, Antioxidant activity, Antimicrobial Activity, DPPH, HR-LCMSItem Study Of Ethnoecology And Phytosociology Of Vegetation With Its Use Value And Conservation Status Of Bhanu Municipality, Central Nepal.(Amrit Campus, 2023-09-29) Dinesh Acharya; Dr. Laxmi Joshi ShresthaAvailable Full TextItem Effect Of Ageratina Adenophora Invasion On Litter Decomposition, Fine Root Production And Mycelial Growth Of Selected Saprophytic Fungi(Amrit Campus, 2024-06-30) Deepa Gharti Magar; Dr. Laxmi Joshi ShresthaAvailable Full TextItem Traditional Knowledge To Treat Maternal And Child Health Illness In Runtigadhi Rural Municipality, Rolpa District, Lumbini Province, Western, Nepal(Amrit Campus, 2023) Anisha Bhandari; Dr. Shila SinghAvailable Full Text